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Rodent Auditory Brainstem Response Test
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Rodent Auditory Brainstem Response Test

See more by: MazeEngineers

$890.00 – $24,850.00

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Description

The Maze Engineers Rodent Auditory Brainstem Response (ABR) Test kit is designed to evaluate the auditory pathway’s function in rodents. This neurophysiological assessment involves presenting clicks or brief tone bursts to the rodent’s ear, while electrodes positioned on the skin over the brainstem record the electrical activity generated. By analyzing these responses, researchers can determine the health of the auditory nerve and various brainstem nuclei. The ABR test is widely used to measure hearing thresholds in rodents and can also identify auditory pathway damage or assess the impact of drugs and other treatments on auditory function.

Maze Engineers provides the Rodent Auditory Brainstem Response Test.

Meet the producer

Producer: MazeEngineers

MazeEngineers offers custom-built behavioral mazes at no extra cost—designed to fit your exact research needs. Eliminate reproducibility issues from poor sizing or lingering scent cues with precision-engineered, modular, and smart mazes that adapt in real time to animal behavior. Publish new protocols, run adaptive experiments, and push the boundaries of behavioral science.

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Frequently bought together

Description

Conduct ABR software

Conduct ABR Maze software

Maze Engineers Sound System

Maze Engineers control box

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Electronic controller and sound generator

Brain response recorder

TDT Rz6 Multi-I/O high-frequency auditory processor

Mouse Auditory Brainstem Response Test

Isolation Cubicle

Animal enclosure

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TDT RZ6 Multi-I/O high-frequency auditory processor

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Features

Introduction

Auditory Brainstem Response (ABR) testing was pioneered in humans by Jewett and Williston during the late 1960s (Jewett & Williston, 1971). This neurophysiological test is instrumental in researching and diagnosing neural disorders and assessing hearing sensitivity (Galambos & Hecox, 1978; Sohmer, Freeman, Friedman, & Lidan, 1991). In animal studies, auditory stimuli are frequently used in behavioral assays such as the Fear Conditioning Assay and Sociability Chamber. Variations in hearing sensitivity among the subjects can notably influence their behavior and the results observed. Although vocalization isn’t the main mode of communication in animals, it remains a crucial aspect of human interaction and quality of life.

ABR recordings involve placing electrodes near the subject’s scalp to capture evoked potentials. When sound stimuli are presented, the auditory brainstem pathways—including cochlear ganglion neurons and their associated fiber tracts and nuclei—generate electrical responses that provide insights into the health of these neural structures. The ABR waveform typically comprises five distinct components, labeled I to V, reflecting the activation of the auditory nerve and the processing of sound through the brainstem pathways.

Apparatus and Equipment

The Rodent Audible Brainstem Response (ABR) Test setup features a soundproof isolation chamber with an animal enclosure, equipped with LED and infrared lighting. An external device can be connected through a customizable device slot, and a camera mount is positioned at the top of the chamber for observation.

The Maze Engineers control box interfaces with the TDT RZ6 processor to deliver TTL signals that synchronize with each stimulus presentation.

Sound stimuli are produced by a generator with adjustable parameters, and are played through two speakers capable of emitting tones or white noise. A sound meter is used to monitor and ensure accurate sound levels.

The rodent’s auditory neural pathway generates electrical responses to these stimuli, which the TDT RZ6 then records in high fidelity, capturing neural activity, stimulus waveforms, and related behavioral events.

The Conduct ABR Maze software enables the creation of customized sound stimuli protocols, allowing adjustments to parameters such as frequency, dB level, phase, and duration.

Protocol

Follow appropriate laboratory protocols, surgical hygiene, and animal welfare practices before commencing. Clean and sterilize all surgical equipment and other apparatus.

The following protocol considers a mouse as the subject. The protocol can be applied to other small rodents as well.

Data Analysis

These data recored from the rodent auditory brainstem response apparatus can be used to evaluate hearing function, diagnose hearing disorders, study the effects of ototoxic drugs or noise exposure on the auditory system, assess the efficacy of hearing protection devices or therapies, and investigate the neural mechanisms of hearing and auditory processing.

  1. Hearing threshold: The minimum sound intensity required to elicit an ABR waveform. This is an objective measure of hearing sensitivity.
  2. Latency: The time interval between the onset of the acoustic stimulus and the occurrence of the corresponding ABR peak. This provides information about the speed of neural conduction along the auditory pathway.
  3. Amplitude: The magnitude of the ABR peak, which reflects the number of synchronized firing of auditory neurons. This can indicate the integrity of the auditory pathway.
  4. Waveform morphology: The shape of the ABR waveform, which can reveal information about the functional organization of the auditory system and the type of hearing loss.
  5. Interwave interval: The time interval between successive ABR peaks, which can provide insights into the timing and processing of auditory information.
  6. Signal-to-noise ratio: The difference between the amplitude of the ABR response and the background noise level, which reflects the quality of the ABR recording and the reliability of the measurement.

Literature Review

Recommendations, Considerations, and Precautions

  • Prior to anesthetization of the subjects, calibrate, program, and check all devices and equipment for proper functioning.
  • Use a Faraday cage inside the sound-proof chamber to prevent interference from external electronic devices. 
  • Use a non-electric heating pad during ABR testing to avoid any interferences during recordings.
  • To prevent the possibility of the subject waking up during ABR recording sessions (approximately 40-minute sessions), inject one-fifth of the original dose of the anesthetic at about 20 minutes during testing.
  • Provide appropriate post-experimental recovery and pain management to the subjects following ABR recordings.

References

  1. Akil, O., Oursler, A. E., Fan, K., & Lustig, L. R. (2016). Mouse Auditory Brainstem Response Testing. Bio-protocol, 6(6), e1768. https://doi.org/10.21769/BioProtoc.1768 
  2. Akil, O., Sun, Y., Vijayakumar, S., Zhang, W., Ku, T., Lee, C. K., … & Lustig, L. R. (2015). Spiral ganglion degeneration and hearing loss as a consequence of satellite cell death in saposin B-deficient mice. Journal of Neuroscience, 35(7), 3263-3275.
  3. Galambos, R., & Hecox, K. E. (1978). Clinical applications of the auditory brain stem response. Otolaryngologic Clinics of North America, 11(3), 709-722.
  4. Sohmer, H., Freeman, S., Friedman, I., & Lidan, D. (1991). Auditory brainstem response (ABR) latency shifts in animal models of various types of conductive and sensori-neural hearing losses. Acta oto-laryngologica, 111(2), 206–211. https://doi.org/10.3109/0001648910913737
  5. Jewett, D. L., & Williston, J. S. (1971). Auditory-evoked far fields averaged from the scalp of humans. Brain, 94(4), 681-696.
  6. Lundt, A., Soos, J., Henseler, C., Arshaad, M. I., Müller, R., Ehninger, D., … & Weiergräber, M. (2019). Data acquisition and analysis in brainstem evoked response audiometry in mice. JoVE (Journal of Visualized Experiments), (147), e59200.
  7. O’Leary, T. P., Shin, S., Fertan, E., Dingle, R. N., Almuklass, A., Gunn, R. K., … & Brown, R. E. (2017). Reduced acoustic startle response and peripheral hearing loss in the 5xFAD mouse model of Alzheimer’s disease. Genes, Brain and Behavior, 16(5), 554-563.
  8. Liu, Y., Fang, S., Liu, L. M., Zhu, Y., Li, C. R., Chen, K., & Zhao, H. B. (2020). Hearing loss is an early biomarker in APP/PS1 Alzheimer’s disease mice. Neuroscience letters, 717, 134705.
  9. Huang, S., Xu, A., Sun, X., Shang, W., Zhou, B., Xie, Y., … & Han, F. (2020). Otoprotective Effects of α-lipoic Acid on A/J Mice with Age-related Hearing Loss. Otology & Neurotology, 41(6), e648-e654.

Additional information

Rodent Auditory Brainstem Response Test

Brain response recorder, Conduct ABR software, Maze Engineers Sound System, Mouse Auditory Brainstem Response Test

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